Job Description

Job Title: Research Associate
Location: Sapien Biosciences, Hyderabad
Reporting to: Senior Scientist
Position Overview:
This is a junior-level, applied, and clinically oriented research position at Sapien Biosciences,
Hyderabad. The selected candidate will support laboratory-based research involving human
tissues, blood-derived cells, and molecular assays. The role requires a minimum of 1 year of
hands-on experience in mammalian cell culture and related laboratory techniques, along with
strong documentation and organizational skills.
Key Responsibilities:
All these activities and responsibilities listed below are generally part of the scientific projects
handled by Sapien and will be performed as per project requirements, wherever applicable
A) Cell Culture & Microscopy

• Establish and maintain primary human cell cultures and established cell lines from
  tissues and blood samples.
• Perform routine QC assays, including mycoplasma testing.
• Assist in light microscopy, immunofluorescence, and flow cytometry (FACS) for cell
  characterization as and when required for the projects.
  B) Patient Sample Processing
• Isolate lymphocytes (e.g., PBMCs) and other cell populations from human blood and
  other samples.
  C) Drug & Biomarker Evaluation
• Conduct in vitro assays to evaluate drugs, biomarkers, and formulations using human
  skin tissue, and 2D/3D cell culture models in 96- and 384-well formats.
• Perform assays for cell proliferation, cytotoxicity, ELISA, and stem cell marker
  expression.
• Record and analyze experimental results; prepare data summaries, reports, and
  presentations using Excel and PowerPoint templates.
  D) Histology & Molecular Biology
• Support IHC and ISH experiments (experience preferred).
• Isolate DNA, RNA, and protein from tissues, blood, and cultured cells.
• Perform PCR assays for selected genes as per project requirements.
  
  E) Documentation, Inventory & Teamwork
• Maintain accurate and up-to-date SOPs, experimental records, and lab notebooks in
  compliance with lab and client quality standards.
• Manage laboratory inventory, including tracking and replenishment of reagents,
  consumables, and supplies.
• Work collaboratively with the scientific and biobank teams for experiment planning,
  literature review, and workflow coordination.
  Essential Qualifications and Experience:
• M.Sc. / M.Tech in Life Sciences, Biotechnology, Biochemistry, Molecular Biology, or
  a related Life Science discipline.
• Minimum 1 year of hands-on experience in mammalian cell culture and associated
  laboratory techniques.
• Practical experience with molecular biology assays, immunoassays, and cell-based
  experiments.
• Knowledge of inventory management and basic laboratory operations.
• Strong organizational, documentation, and communication skills.
• Ability to work efficiently as part of a collaborative research team.
  Desired Qualifications and Skills:
• Experience in cell-based assays including viability, proliferation, and cytotoxicity
  studies.
• Knowledge of cell counting, plating, and assay setup in 96- and 384-well formats.
• Working knowledge of molecular biology and protein quantification techniques.
• Experience with immunohistochemistry (IHC), ELISA, and PCR assays.
• Hands-on experience in handling primary cultures and processing patient-derived
  samples.
• Familiarity with sterile techniques and biosafety practices for handling human-derived
  materials
  Employment Type
  Full-time, on-site Hyderabad
  Suitable candidates are invited to send their updated CVs to Email: hr@sapienbio.com