Job Description

Research Technician Role

About Persephoni: Persephoni Bio is a Venture Studio dedicated to building and accelerating next-generation therapeutics. We partner with exceptional scientific founders and academic innovators to translate breakthrough research into transformative medicines across oncology, neurology, immunology, and regenerative medicine. Our model combines deep scientific rigor, disciplined capital deployment, and hands-on operational support to systematically de-risk and advance early-stage biotech companies. For more information visit www.persephonibio.com.

We are seeking an experienced and motivated Research Technician to join our discovery lab in Durham, and contribute to cutting-edge experimental work supporting multiple therapeutic programs. The ideal candidate will bring strong technical expertise, scientific curiosity, and a proactive mindset to drive high-quality lab execution in a collaborative environment. Below is a job description outlining the desired qualifications and key responsibilities for this role.

Location: Durham, NC (RTP) – Onsite | Local candidates are encouraged to apply

Employment Type: Full-Time, 6-month contract (with potential conversion)

Compensation range: $30–$38 per hour, commensurate with experience

Classification: Non-exempt (hourly)

Benefits: Not applicable

Work Authorization: Must be authorized to work in the United States

Applicants should be comfortable performing the majority of these responsibilities independently.

• Mammalian cell culture (BSL-2)
• RNA extraction and handling at scale
• RT-qPCR execution, troubleshooting, and data analysis
• Experimental documentation and data reporting

Position Overview

Persephoni BioPartners is seeking a motivated Research Technician / Research Associate to support discovery-stage research in our RTP laboratory. This is a hands-on bench role focused on executing cell culture and RNA-based molecular biology workflows that generate high-quality, decision-driving data across multiple therapeutic programs. The Technician will work closely with scientists and program managers in a fast-paced, collaborative environment typical of a venture-backed discovery organization.

This role is not focused on animal work, GMP manufacturing, or clinical laboratory operations.

Key Skills

Cell Culture & Molecular Biology

• Maintain mammalian cell cultures (cell lines and primary cells) using aseptic technique (BSL-2)
• Perform routine passaging, cell counting, treatments, screening, and cryopreservation
• Execute RNA extraction, cDNA synthesis, and RT-qPCR for multiple targets and samples
• Operate and troubleshoot qPCR systems, thermal cyclers, plate readers, and related equipment
• Collaborate with scientists to optimize protocols and resolve experimental issues

Data Analysis & Documentation

• Collect, organize, and analyze experimental data (e.g., ΔΔCt calculations)
• Generate tables and figures using Excel and/or GraphPad Prism
• Maintain accurate, industry-grade experimental records in an electronic lab notebook (ELN)

Laboratory Operations & Safety

• Prepare and maintain reagents, buffers, and media
• Ensure tissue culture and laboratory spaces are clean, stocked, and functional
• Follow all biosafety, chemical safety, and waste disposal protocols

Qualifications

Education

• Bachelor’s degree in Molecular Biology, Cell Biology, Biochemistry, or a related science field required
• Master’s degree preferred

Experience & Technical Skills

• 2–4+ years of hands-on laboratory experience in academic or industry research settings (industry experience preferred)
• Strong proficiency in mammalian cell culture
• Experience with RNA isolation and RT-PCR or qPCR
• Familiarity with RNA handling best practices and RNase-free techniques
• Experience generating and interpreting primary experimental data (not just running protocols)
• Experience with ELNs and structured record-keeping preferred

Data & Analysis

• Comfortable analyzing experimental data using Excel and/or GraphPad Prism
• Ability to perform basic calculations, generate plots, and interpret results
• Understanding of basic statistical concepts (mean, standard deviation, etc.)

Additional Skills (Nice to Have)

• Primer design or sequencing data interpretation
• siRNA or oligonucleotide-based experiments
• Flow cytometry, fluorescent imaging, or molecular cloning

These skills are not required but may be developed on the job.

Soft Skills

• Strong attention to detail and organization
• Clear written and verbal communication skills
• Ability to manage multiple tasks and work collaboratively in a team environment

Equal Employment Opportunity, Compensation, and Work Authorization

Persephoni Bio is an Equal Opportunity Employer. We do not discriminate on the basis of race, color, religion, sex (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity or expression, national origin, age, disability, genetic information, veteran status, or any other characteristic protected by applicable federal, state, or local law. Compensation is determined based solely on relevant experience, skills, and qualifications, and may vary accordingly. Employment is contingent upon authorization to work in the United States. Persephoni participates in the E-Verify program as required by law. This position is classified as non-exempt and is eligible for overtime pay in accordance with applicable wage and hour laws. Employment with Persephoni is at will. Offers of employment are contingent upon successful completion of applicable pre-employment screenings, which may include background checks, reference checks, and verification of credentials, in compliance with applicable law. Persephoni is a drug-free workplace in accordance with applicable federal and state laws.

Requirements added by the job poster

• Accept a background check

• Working in an onsite setting

• Authorized to work in the United States

• No need for visa sponsorship