JUNIOR SCIENTIST / RESEARCH ASSOCIATE

Job Description

WE ARE HIRING | JUNIOR SCIENTIST / RESEARCH ASSOCIATE Cloning & Cell Line Development | Biologics Kwality Pharmaceuticals Limited is looking for passionate and technically skilled professionals to join our team in the field of Cloning & Cell line development. 📌 Department: Biologics (Cell line development) 📌 Experience: 4–6 Years 📌 Qualification: M.Sc. / M.Tech. in Biotechnology, Molecular Biology. Key Roles and Responsibilities 1.Perform molecular cloning activities, including gene amplification, plasmid preparation, restriction digestion, ligation and recombinant DNA construct preparation. 2.Carry out PCR, colony PCR, agarose gel electrophoresis and DNA purification. 3.Support gene synthesis/sequence verification, plasmid mapping and construct confirmation. 4.Maintain mammalian cell cultures using aseptic techniques and follow good cell culture practices & perform competent cell transformation. 5.Support transfection of mammalian host cells (e.g., CHO-K1, CHO-S, CHO-DG44 and/or HEK293) with recombinant expression constructs. 6.Assist in development, selection and screening of stable cell pools and monoclonal cell lines. 7.Perform single-cell cloning or limiting dilution, where applicable, and maintain cell line records. 8.Monitor cell growth, viability, cell density and culture performance. 9.Support screening of recombinant protein or mAb-producing clones using suitable analytical assays. 10.Maintain cell banks, including master and working cell bank preparation, where applicable. Required Technical Skills 1.Hands-on knowledge of molecular biology and gene cloning techniques. 2.Good understanding of DNA, RNA, genes, vectors, promoters and recombinant DNA technology. 3.Practical knowledge of PCR, restriction digestion, ligation, transformation and plasmid isolation. 4.Basic understanding of mammalian cell culture and aseptic handling techniques. 5.Basic understanding of transfection methods and stable cell line generation. 6.Ability to monitor cell growth, viability and culture conditions. 7.Basic knowledge of recombinant proteins and monoclonal antibody expression. Preferred Skills 1. Experience in CHO and/or HEK293 cell culture and stable cell line development for recombinant proteins or mAbs. 2. Familiarity with cell counting and viability assessment using an automated cell counter or equivalent method. 3.Understanding of cell banking, cryopreservation and thawing procedures. 4. Instruments: Cell Counter,Bioanalyzer, Bioreactor,FACS (Fluorescence-Activated Cell Sorter), Nano Drop Spectrophotometer, PCR Thermal Cycler, Gel Electrophoresis. 📩 Interested candidates may share their updated CV at: career@kwalitypharma.com & hrd@kwalitypharma.com or on whatsap +91-9837454653