Research Scientist/Senior Research Scientist

Job Description

We are Hiring!!! Position: Research Scientist/Senior Research Scientist – In Vitro Biochemical Assay Design & Optimization, Assay Development & Screening, The role is responsible for designing, optimizing, validating, and executing biochemical and biophysical assays to screen small molecules, biologics, or recombinant proteins against therapeutic drug targets (02 Vacancies) Department: In Vitro Biochemical Assay Design & Optimization, High-Throughput & Medium-Throughput Screening (HTS / MTS), High-Throughput Instrumentation & Automation Experience: 2 – 4 / 5-8 years Location: Bommasandra, Bengaluru, Karnataka, India Qualifications: M.Sc/M.Tech (Biochemistry/Biotechnology/Microbiology) Interested candidates can share their resume at sreddy@bachwellpharma.com (main ID), hr@bachwellpharma.com, bachwellpharma@849ayushi

Core Job Responsibilities

1. In Vitro Biochemical Assay Design & Optimization
   Assay Development: Design and establish robust in vitro enzymatic, binding, and functional assays using various detection technologies (e.g., Absorbance, Fluorescence, TR-FRET, FP, Luminescence, AlphaLISA, and SPR).
   Target Characterization: Characterize target enzymes, kinases, proteases, or receptor proteins by determining kinetic parameters (K_m, V_{max}, k_{cat}) and binding affinity parameters (K_d, IC_{50}, EC_{50}).
   Parameter Tuning: Systematically optimize reaction conditions, including enzyme/substrate concentrations, incubation times, buffer composition, pH, salt strength, detergents, and DMSO tolerance to achieve robust signal-to-background ratios and low variability.
2. High-Throughput & Medium-Throughput Screening (HTS / MTS)
   Compound Screening: Execute primary, secondary, and hit-validation screening campaigns (in 96-, 384-, or 1536-well microplate formats) for synthetic chemical libraries, natural products, or biologic drug candidates.
   Assay Robustness:
   Mechanism of Action (MoA) Studies:
3. High-Throughput Instrumentation & Automation
   Plate Readers & Detection Systems: Operate, calibrate, and maintain multi-mode microplate readers (e.g., EnVision, PHERAstar, SpectraMax, CLARIOstar).Automated Liquid Handlers: Program and utilize automated liquid handling workstations (e.g., Tecan Freedom EVO/Fluent, Hamilton STAR, ECHO Acoustic Liquid Handler, Agilent Bravo) for serial dilutions and automated plate prep.

Equipment Maintenance:
4. Data Analysis, Informatics & ReportingQuantitative Analysis: Process raw screening data using specialized curve-fitting and assay informatics software (e.g., GraphPad Prism, ActivityBase, Dotmatics, CDD Vault) to derive $IC_{50}$ / $EC_{50}$ curves and hill slopes.
Data Integrity & Documentation:
Cross-Functional Communication:
5. Laboratory Management & SOP Maintenance
SOP Authoring: Draft, update, and review technical SOPs, validation protocols, and assay transfer guidelines.
Reagent Procurement & Quality:
Lab Safety & Compliance: